Characterization of Genetic Polymorphism of the Bovine Lymphocyte Antigen DRB3.2 Locus in Sistani Cattle of Iran (Bos indicus)
نویسندگان
چکیده
منابع مشابه
Distribution of bovine lymphocyte antigen (BoLA-DRB3) alleles in Brazilian dairy Gir cattle (Bos indicus).
Brazilian dairy Gir (Bos indicus) cattle are a tropical, well-adapted breed, for which no information on the major histocompatibility complex (MHC) is presently available. The second exon of the bovine lymphocyte antigen (BoLA-DRB3) was amplified by polymerase chain reaction (PCR) of DNA samples from 28 Brazilian dairy Gir cows. Two experimental genotyping approaches were used: direct sequencin...
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in current study, 63 samples of bat populations collected from differ regions were used for evaluating the geographic variations. twenty cranial and dental characters for traditional morphometric and landmarks method on the ventral, dorsal skull and mandible for geometry morphometric studies were used. statistical analyses of traditional morphometric and geometry morphometric data indicated low...
Polymorphism of Bovine Lymphocyte Antigen DRB3.2 in Holstein Bulls of Iran Using PCR-RFLP
The Holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (BoLA-DRB3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was extracted from bull semen using phenol-chloroform method. A two-step PCR was conducted in order to amplify a 284 base-pair fragment of the target gene. Amplicons were digested by RsaI, HaeIII and BstyI ...
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ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2008
ISSN: 1682-296X
DOI: 10.3923/biotech.2008.333.337